PubMed日本語 - 海洋イガイMytilus galloprovincialisのタンパク質のタンパク質抽出と二次元のゲル電気泳動:タンパク質発現研究、食物質と安全性評価のための重要な道具。―QLifePro医療翻訳医療翻訳 QLifePro


Protein extraction and two-dimensional gel electrophoresis of proteins in the marine mussel Mytilus galloprovincialis: an important tool for protein expression studies, food quality and safety assessment.

海洋イガイMytilus galloprovincialisのタンパク質のタンパク質抽出と二次元のゲル電気泳動:タンパク質発現研究、食物質と安全性評価のための重要な道具。

Published date



Alexandre Campos, Maria Puerto, Ana Prieto, Ana Cameán, André M Almeida, Ana V Coelho, Vitor Vasconcelos


Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR, Universidade do Porto, P-4050-123, Porto, Portugal.


BACKGROUND: Shellfish farming is an important economic activity that provides society with a valuable source of food. Analyses of the protein content and metabolism of shellfish are therefore of utmost importance to monitor the presence and effects of environmental contaminants in these organisms and also to assess food quality and authenticity. The aim of the present study was to compare different protein extraction protocols commonly used in two-dimensional gel electrophoresis (2DE) research and select the most suitable for the analysis of gill and digestive gland proteomes from the marine mussel Mytilus galloprovincialis.


RESULTS: High-resolution protein separation was achieved by direct solubilisation of proteins from M. galloprovincialis tissues with urea (7 mol L(-1)), thiourea (2 mol L(-1)), CHAPS (40 g L(-1)), DTT (65 mmol L(-1)) and ampholytes (pH 4-7, 8 mL L(-1)). Subsequent protein identification from 2DE gels by MALDI-TOF/TOF mass spectrometry revealed a high number of proteins with functions in cytoskeleton structure, dynamics and maintenance. Other proteins identified in the 2DE gels are involved in energy production and carbohydrate metabolism, metal transport, chaperones and stress response, cell signalling and regulation, proteolysis and protein transduction.


CONCLUSION: Important protein markers for contaminant and quality assessment of shellfish food products can be analysed using 2DE.


本研究の目的は、二次元のゲル電気泳動(2DE)研究で一般に使われる異なるタンパク質抽出プロトコルを比較することになっていて、海洋イガイMytilus galloprovincialisから、えらと消化腺プロテオームの分析に最もふさわしいものを選択することになっていた。

結果高解像度タンパク質分離は、尿素(7モルL(-1))、チオ尿素(2モルL(-1))、CHAPS(40g L(-1))、DTT(65mmol L(-1))と両性電解質(pH 4-7、8mLのL(-1))でM. galloprovincialis組織からタンパク質の直接の可溶化によって成し遂げられた。


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