PubMed日本語 - 前駆症状のSOD1 ALSマウスからの脊髄運動ニューロンのRNASeqプロファイリング。―QLifePro医療翻訳医療翻訳 QLifePro


RNA-Seq profiling of spinal cord motor neurons from a presymptomatic SOD1 ALS mouse.

前駆症状のSOD1 ALSマウスからの脊髄運動ニューロンのRNASeqプロファイリング。

Published date



Urmi Bandyopadhyay, Justin Cotney, Maria Nagy, Sunghee Oh, Jing Leng, Milind Mahajan, Shrikant Mane, Wayne A Fenton, James P Noonan, Arthur L Horwich


Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America.


Mechanisms involved with degeneration of motor neurons in amyotrophic lateral sclerosis (ALS; Lou Gehrig's Disease) are poorly understood, but genetically inherited forms, comprising ~10% of the cases, are potentially informative. Recent observations that several inherited forms of ALS involve the RNA binding proteins TDP43 and FUS raise the question as to whether RNA metabolism is generally disturbed in ALS. Here we conduct whole transcriptome profiling of motor neurons from a mouse strain, transgenic for a mutant human SOD1 (G85R SOD1-YFP), that develops symptoms of ALS and paralyzes at 5-6 months of age. Motor neuron cell bodies were laser microdissected from spinal cords at 3 months of age, a time when animals were presymptomatic but showed aggregation of the mutant protein in many lower motor neuron cell bodies and manifested extensive neuromuscular junction morphologic disturbance in their lower extremities. We observed only a small number of transcripts with altered expression levels or splicing in the G85R transgenic compared to age-matched animals of a wild-type SOD1 transgenic strain. Our results indicate that a major disturbance of polyadenylated RNA metabolism does not occur in motor neurons of mutant SOD1 mice, suggesting that the toxicity of the mutant protein lies at the level of translational or post-translational effects.


そして、変異体ヒトSOD1(G85R SOD1-YFP)(ALSの症状を現して、5-6で何ヵ月にも及ぶ年齢を麻痺させる)のためにトランスジェニックである。

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