PubMed日本語 - [骨と骨コラーゲン代謝の生化学指数に対する電気鍼療法の影響と卵巣のない骨粗鬆症モデル・ラットにおけるTNF-α]。―QLifePro医療翻訳医療翻訳 QLifePro


[Effect of electroacupuncture on the biochemical indices of bone and bone collagen metabolism and TNF-alpha in osteoporosis model rats without ovaries].


Published date


Zhongguo Zhen Jiu. 2012; 32; 1108-12;


Sheng-Yong Bao, Shao-Jun Zhang, Wan-Juan Lin, Jing-Fen Chen


Department of Physiotherapy, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China.


OBJECTIVE: To explore the mechanisms of electroacupuncture (EA) on postmenopausal osteoporosis (PMO).


METHODS: Sixty female SD rats aged 6 months were selected, resected double ovaries and fed for 90 days in order to make the model of experimental osteoporosis, and then, they were randomly divided into a model control group (without any therapy), a routine acupoints applying group, which were treated with EA at "Pishu" (BL 20), "Weishu" (BL 21), "Shenshu" (BL 23) and "Qihaishu" (BL 24), and an EA with tonification method (EAT) group at "Guanyuan" (CV 4) and "Zusanli" (ST 36), and an EA with dispersing blood stasis method (EAD) group at "Shenshu" (BL 23), "Geshu" (BL 17) and "Dazhu" (BL 11), and an EA with tonification and dispersing blood stasis method (EATD) group at "Guanyuan" (CV 4), "Zusanli" (ST 36), "Shenshu" (BL 23), "Geshu" (BL 17) and "Dazhu" (BL 11), 12 rats in each group. EA therapy was performed once a day, 30 min each time. Then these rats rested for 1 day after consecutive treatment for 6 days. The treatment lasted for 12 weeks. Enzyme linked immunosorbent assay (ELISA) was used to examine the level of TNF-alpha, and the level of E2, osteocalin (BGP) and carboxyterminal propeptide of type I procollagen (PICP), carboxyterminal telopeptide of type I lollagen (ICTP) by radioimmunoassay, and alkaline phosphatase by p-nitrophenylphosphate method, and Ca, P, Deoxypyridinoline (DPD) and creatinine (Cr) in urine were detected by automatic biochemistry analyzer.


RESULTS: Compared with model control group, the level of DPD/Cr, Ca/Cr, and the serum contents of ALP, BGP, TNF-alpha and ICTP in all EA groups decreased significantly (all P < 0.01), the level of E2 and PICP in serum increased significantly (all P < 0.01). Compared with EATD group, the level of DPD/Cr and Ca/Cr in the other three EA groups had no statistical differences (all P > 0.05), but the level of ALP, BGP, TNF-alpha and ICTP in serum increased significantly (all P < 0.01), the level of E2 and PICP in serum decreased significantly (all P < 0.01).


CONCLUSION: Acupuncture can reduce significantly the level of DPD/Cr, Ca/Cr, and the serum contents of ALP, BGP, TNF-alpha and ICTP, increase significantly the level of E2 and PICP in PMO model rats and EA with tonification and dispersing blood stasis method is superior significantly to the other routine acupuncture methods.


そして、「Pishu」(BL 20)、「Weishu」(BL 21)、「腎兪」(BL 23)と「Qihaishu」(BL 24)状態にあるEAと「Guanyuan」(CV 4)と「Zusanli」(ST 36)のtonification方法(EAT)群と「腎兪」(BL 23)、「Geshu」(BL 17)と「大竹」(BL 11)状態にあるうっ血方法(EAD)群とtonificationによるEAを分散させて、「Guanyuan」(CV 4)、「Zusanli」(ST 36)、「腎兪」(BL 23)、「Geshu」(BL 17)と「大竹」(BL 11)(各群の12匹のラット)でうっ血方法(EATD)群を分散させることによるEAによるEAで、それは治療された。
結果典型的な対照群と比較して、DPD/Cr、Ca/CrとALP、BGP、TNF-αとすべてのEA群のICTPの血清内容のレベルは有意に減少した(すべてのP < 0.01)。
そして、E2のレベルと血清のPICPが有意に増加した(すべてのP < 0.01)。
EATD群と比較して、DPD/Crのレベルとその他の3つのEA群のCa/Crには、統計差異がなかった(すべてのP > 0.05)、しかし、ALP、BGP、TNF-αと有意に増加する(すべてのP < 0.01)血清、E2のレベルと有意に減少する(すべてのP < 0.01)血清のPICPのICTPのレベル。

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