PubMed日本語 - 定量化と胃腺癌細胞のL-ドーパデカルボキシラーゼ表現の研究は、化学療法物質と交渉した。―QLifePro医療翻訳医療翻訳 QLifePro



Quantification and study of the L-DOPA decarboxylase expression in gastric adenocarcinoma cells treated with chemotherapeutic substances.

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Quantification and study of the L-DOPA decarboxylase expression in gastric adenocarcinoma cells treated with chemotherapeutic substances.


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Dimitrios Korbakis, Emmanuel G Fragoulis, Andreas Scorilas


Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, Athens, Greece.


3,4-Dihydroxy-L-phenylalanine decarboxylase (DDC) is an enzyme implicated in the biosynthetic pathways of the neurotransmitters dopamine and probably serotonin. DDC gene expression has been studied in numerous malignancies and the corresponding data have shown remarkable alterations in the mRNA and/or protein levels encoded by the gene. The aim of this study was to examine any modulations in the DDC mRNA levels in gastric cancer cells after their treatment with the chemotherapeutic agents 5-fluorouracil, leucovorin, irinotecan, etoposide, cisplatin, and taxol. The sensitivity of the AGS gastric adenocarcinoma cells to the antineoplastic drugs was evaluated using the MTT assay. Total RNA was extracted and reverse transcribed into cDNA. A highly sensitive quantitative real-time PCR methodology was developed for the quantification of DDC mRNA. GAPDH was used as a housekeeping gene. Relative quantification analysis was carried out using the comparative C T method ((Equation is included in full-text article.)). The treatment of AGS cells with several concentrations of various broadly used anticancer drugs resulted in significant modulations of the DDC mRNA levels compared with those in the untreated cells in a time-specific and drug-specific manner. Generally, DDC expression levels appeared to decrease after three time periods of exposure to the selected chemotherapeutic agents, suggesting a characteristic DDC mRNA expression profile that is possibly related to the mechanism of each drug. Our experimental data show that the DDC gene might serve as a new potential molecular biomarker predicting treatment response in gastric cancer cells.


本研究の目的は、化学療法剤5‐フルオロウラシル、ロイコボリン、イリノテカン、エトポシド、シスプラチンとtaxolでそれらの治療の後胃癌細胞でDDC mRNAレベルでどんな調節でも調べることになっていた。
非常に感度が高い定量的リアルタイムPCR方法は、DDC mRNAの定量化のために開発された。
相対的な定量化分析は、比較のC T方法((方程式は、フルテキストの冠詞に含まれる。
さまざまな広く使用される抗癌剤のいくつかの濃度によるAGS細胞の処置は、時間に特有および薬物特異性方法で、未処置の細胞でそれらと比較してDDC mRNAレベルの有意の調節に帰着した。
そして、おそらく各薬の機序に関連がある特徴的DDC mRNA発現プロフィールを示唆した。

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