PubMed日本語 - 水産養殖でプロバイオティクスとして意図される水生の起源の乳酸菌の抗菌活性、抗生感染性と毒性因子。―QLifePro医療翻訳医療翻訳 QLifePro


Antimicrobial activity, antibiotic susceptibility and virulence factors of Lactic Acid Bacteria of aquatic origin intended for use as probiotics in aquaculture.


Published date



Estefanía Muñoz-Atienza, Beatriz Gómez-Sala, Carlos Araújo, Cristina Campanero, Rosa del Campo, Pablo E Hernández, Carmen Herranz, Luis M Cintas


Grupo de Seguridad y Calidad de los Alimentos por Bacterias Lácticas, Bacteriocinas y Probióticos (Grupo SEGABALBP) Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, 28040, Spain.


BACKGROUND: The microorganisms intended for use as probiotics in aquaculture should exert antimicrobial activity and be regarded as safe not only for the aquatic hosts but also for their surrounding environments and humans. The objective of this work was to investigate the antimicrobial/bacteriocin activity against fish pathogens, the antibiotic susceptibility, and the prevalence of virulence factors and detrimental enzymatic activities in 99 Lactic Acid Bacteria (LAB) (59 enterococci and 40 non-enterococci) isolated from aquatic animals regarded as human food.


RESULTS: These LAB displayed a broad antimicrobial/bacteriocin activity against the main Gram-positive and Gram-negative fish pathogens. However, particular safety concerns based on antibiotic resistance and virulence factors were identified in the genus Enterococcus (86%) (Enterococcus faecalis, 100%; E. faecium, 79%). Antibiotic resistance was also found in the genera Weissella (60%), Pediococcus (44%), Lactobacillus (33%), but not in leuconostocs and lactococci. Antibiotic resistance genes were found in 7.5% of the non-enterococci, including the genera Pediococcus (12.5%) and Weissella (6.7%). One strain of both Pediococcus pentosaceus and Weissella cibaria carried the erythromycin resistance gene mef(A/E), and another two P. pentosaceus strains harboured lnu(A) conferring resistance to lincosamides. Gelatinase activity was found in E. faecalis and E. faecium (71 and 11%, respectively), while a low number of E. faecalis (5%) and none E. faecium exerted hemolytic activity. None enterococci and non-enterococci showed bile deconjugation and mucin degradation abilities, or other detrimental enzymatic activities.


CONCLUSIONS: To our knowledge, this is the first description of mef(A/E) in the genera Pediococcus and Weissella, and lnu(A) in the genus Pediococcus. The in vitro subtractive screening presented in this work constitutes a valuable strategy for the large-scale preliminary selection of putatively safe LAB intended for use as probiotics in aquaculture.


しかしながら、抗生物質耐性と毒性因子に基づく特定の安全懸念は、膝エンテロコッカス(86%)(エンテロコッカスフェカーリス、100%; E. faecium、79%)で同定された。
ペジオコックス属pentosaceusとWeissella cibariaの1つの株はエリスロマイシン抵抗性遺伝子mef(1/E)を運んだ、そして、さらに2つのP. pentosaceus株はリンコサミドに抵抗をもたらしているlnu(A)を保菌した。
低い数のE. faecalis(5%)と全くE. faeciumでないのが溶血性活性を振るう間、ゼラチナーゼ活性はE. faecalisとE. faecium(それぞれ71と11%)で見つかった。

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