PubMed日本語 - Carbon MonoxideによるIron-Deficiency Responses Is Associatedの遺伝子Regulationとクラミドモナス属reinhardtiiのHeme Oxydase 1。―QLifePro医療翻訳医療翻訳 QLifePro



Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii.

Published date 2013 Jan 22

Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii.

Carbon MonoxideによるIron-Deficiency Responses Is Associatedの遺伝子Regulationとクラミドモナス属reinhardtiiのHeme Oxydase 1。

Published date



Zhang Liping, Shao Hongbo, Long Xiaohua, Liu Zhaopu


Jiangsu Key Lab of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China ; Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Costal Zone Research(YIC), Chinese Academy of Sciences (CAS), Yantai, China.


Carbon monoxide (CO) as an endogenous gaseous molecule regulates a variety of biological processes in animals. However, CO regulating nutrient stress responses in green alga is largely unknown. On the other hand, heme oxydase (HO1 as a rate-limiting enzyme of the first step for heme degration and to catalyze heme into biliverdin (BV), which is concomitant with releasing of CO and ferrous ions, probably participates in the process of CO-regulating response to nutrient stress in green alga. In this paper, we described an observation that CO could regulate iron-homeostasis in iron-starving Chlamydomonas reinhardtii. Exogenous CO at 8 µM was able to prevent the iron deficient-inducing chlorosis and improve chlorophyll accumulation. Expression pattern of FOX1, FTR1 and ferredoxin was up-regulated by CO exposure in iron-deficient mediam. treatment with external CO increasing iron accumulation in iron-deficient C. reinhardtii. Moreover, to get insights into the regulatory role of HO1, we constructed a transgenic alga overexpressing HO1 and HO1 knock-out mutants. The results show that there was no significant influence on chlorosis with HO1 overexpression of C. reinhardtii under iron-deficiency and the chlorophyll accumulation, and gene expression associated with iron deficiency of mutant were greatly improved. Otherwise, those results from HO1 knock-out mutants were opposite to HO1 overexpression mutants. Finally, CO exposure induced NO accumulation in cells. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO/HO1 may play an important role in improving green algae adaptation to iron deficiency or cross-talking with NO under the iron deficiency.


外部のCOが鉄欠乏性C. reinhardtiiで鉄の蓄積を増加させる治療。
成績は萎黄病に対する有意の影響が鉄欠乏と葉緑素蓄積の下にC. reinhardtiiのHO1過剰発現でなかったことを示す、そして、変異体の鉄欠乏と関連した遺伝子発現は非常に改善された。

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